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Novel Experimental Mouse Model to Study Malaria-Associated Acute Kidney Injury.

Johanna Bensalel1,2, Alexandra Roberts1, Kiara Hernandez1

  • 1Department of Biological Sciences, Bronx, Lehman College, The City University of New York, New York, NY 10468, USA.

Pathogens (Basel, Switzerland)
|April 28, 2023
PubMed
Summary

Researchers developed a novel mouse model for malaria-associated acute kidney injury (MAKI) by performing unilateral nephrectomies before infection. This model effectively replicates human MAKI pathology, aiding future research into this critical condition.

Keywords:
AKIMAKIacute kidney injurymalariarenal impairmentsevere malariasevere malaria mouse model

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Area of Science:

  • Nephrology
  • Infectious Diseases
  • Experimental Pathology

Background:

  • Malaria-associated acute kidney injury (MAKI) is a severe complication of malaria, significantly increasing mortality in children.
  • Research and effective experimental models for MAKI are lacking, hindering understanding and treatment development.

Purpose of the Study:

  • To develop a standardized in vivo mouse model that accurately reflects the pathology observed in human MAKI patients.
  • To provide a crucial tool for investigating the mechanisms, diagnosis, and treatment of MAKI.

Main Methods:

  • Wild-type mice underwent unilateral nephrectomy (surgical removal of one kidney).
  • Mice were subsequently infected with *Plasmodium berghei* NK65.
  • Kidney injury was assessed through histopathological analysis and measurement of AKI biomarkers (urinary NGAL, serum Cystatin C, BUN).

Main Results:

  • Nephrectomized mice infected with *P. berghei* NK65 exhibited significant kidney injury compared to non-nephrectomized controls.
  • Elevated levels of key AKI biomarkers were observed in the infected, nephrectomized mice.
  • The model successfully replicated common pathological findings seen in human MAKI.

Conclusions:

  • The established unilateral nephrectomy and *P. berghei* NK65 infection model is a viable tool for studying MAKI.
  • This model will facilitate research into MAKI's molecular pathways, disease progression, and the evaluation of potential adjunctive therapies.