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Related Experiment Video

Updated: Aug 1, 2025

Establishment and Characterization of UTI and CAUTI in a Mouse Model
08:40

Establishment and Characterization of UTI and CAUTI in a Mouse Model

Published on: June 23, 2015

20.2K

Development of a High-Throughput Urosepsis Mouse Model.

Roman Herout1,2, Sreeparna Vappala3,4, Sarah Hanstock1

  • 1The Stone Centre at Vancouver General Hospital, Department of Urologic Sciences, University of British Columbia, Vancouver, BC V6H 3Z6, Canada.

Pathogens (Basel, Switzerland)
|April 28, 2023
PubMed
Summary

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Microsystems & nanoengineering·2026

Researchers developed a new murine model for studying urinary tract infections (UTIs) and sepsis. This model uses a single bacterium, Proteus mirabilis, to mimic slow-progressing urosepsis without high mortality, aiding research into prolonged infections.

Area of Science:

  • Microbiology
  • Immunology
  • Urology

Background:

  • Murine sepsis models are often polymicrobial and associated with high mortality.
  • A need exists for models that mimic slow-paced, monomicrobial sepsis, particularly originating from the urinary tract.

Purpose of the Study:

  • To develop a high-throughput murine model for studying monomicrobial urosepsis.
  • To create a model that mimics a slow-paced infection without rapid mortality, allowing for the study of prolonged sepsis.

Main Methods:

  • Ultrasound-guided percutaneous bladder catheterization in C57Bl/6 mice.
  • Introduction of Proteus mirabilis (PM) into the bladder to establish monomicrobial urosepsis.
  • Assessment of bacterial load, cell-free DNA, D-dimer, and cytokine/chemokine profiles in blood and tissues.
Keywords:
catheter-associated urinary tract infectionmouse modelurosepsis

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Related Experiment Videos

Last Updated: Aug 1, 2025

Establishment and Characterization of UTI and CAUTI in a Mouse Model
08:40

Establishment and Characterization of UTI and CAUTI in a Mouse Model

Published on: June 23, 2015

20.2K
Transurethral Induction of Mouse Urinary Tract Infection
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Transurethral Induction of Mouse Urinary Tract Infection

Published on: August 5, 2010

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A Murine Model of Irreversible and Reversible Unilateral Ureteric Obstruction
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A Murine Model of Irreversible and Reversible Unilateral Ureteric Obstruction

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Main Results:

  • All mice survived the 4-day study period, indicating a non-lethal model.
  • Significant increases in plasma cell-free DNA, D-dimer, and pro-inflammatory cytokines (IFN-γ, IL-6, IP-10, MIG, G-CSF) were observed in infected mice.
  • High bacterial loads were found in urine, on catheters, and in splenic tissue, confirming septicemia.

Conclusions:

  • A reproducible, monomicrobial murine model of urosepsis was successfully developed.
  • This model does not cause rapid deterioration or death, making it suitable for studying prolonged urosepsis.
  • The model allows for investigation of host responses, including inflammation and coagulation, during sustained urosepsis.