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RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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Related Experiment Video

Updated: Aug 1, 2025

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Multicomponent Lipid Nanoparticles for RNA Transfection.

Nataliya Gretskaya¹, Mikhail Akimov¹, Dmitry Andreev¹

¹Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.

|April 28, 2023

Summary

Researchers developed new cationic lipid nanoparticles (LNPs) for efficient RNA delivery. LNPs with DOTAP and GM3 gangliosides showed superior mRNA and siRNA transfection in cancer cells.

Keywords:

GM3 ganglioside cationic lipids lipid nanoparticles mRNA transfection siRNA transfection

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Published on: January 20, 2023

Area of Science:

Biotechnology
Nanomedicine
Molecular Biology

Background:

Cationic lipid nanoparticles (LNPs) are crucial for nucleic acid delivery.
Optimizing LNP composition remains vital for enhanced transfection efficiency.

Purpose of the Study:

To develop novel multi-component cationic LNPs for mRNA and siRNA delivery.
To evaluate the transfection efficiency of LNPs using DOTAP and oleoylcholine (Ol-Ch).
To investigate the role of GM3 gangliosides in LNP-mediated gene delivery.

Main Methods:

Preparation of multi-component LNPs using a three-stage procedure.
Characterization of LNP size (average 176 nm, PDI 0.18).
Assessment of LNP transfection efficiency in MDA-MB-231, SW 620, and HEK 293T cells.

Main Results:

LNPs with DOTAP mesylate outperformed those with Ol-Ch.
Bilayer LNPs exhibited higher transfection activity than core LNPs.
Phospholipid type influenced transfection in cancer cells but not HEK 293T cells.
GM3 ganglioside-containing LNPs demonstrated superior mRNA delivery to MDA-MB-231 and siRNA delivery to SW620 cells.

Conclusions:

A novel lipid platform for efficient RNA delivery to mammalian cells was successfully developed.
DOTAP-based LNPs with GM3 gangliosides represent a promising strategy for targeted gene delivery.
LNP composition significantly impacts transfection efficiency, highlighting the importance of tailored formulations.