Utilizing Robust Design to Optimize Composite Bioadhesive for Promoting Dermal Wound Repair

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Wound Healing

Background:

• Catechol-modified bioadhesives release hydrogen peroxide (H2O2) during curing.
• Optimizing H2O2 release is crucial for enhancing bioadhesive performance and therapeutic effects.

Purpose of the Study:

• To tune the H2O2 release profile and adhesive properties of catechol-modified polyethylene glycol (PEG) composites containing silica particles (SiP).
• To evaluate the efficacy of optimized bioadhesives in promoting wound healing.

Main Methods:

• A robust design experiment using an L9 orthogonal array was employed to investigate the influence of PEG architecture, PEG concentration, phosphate-buffered saline (PBS) concentration, and SiP concentration.
• Adhesive formulations were selected based on predicted H2O2 release (40-80 µM) and tested in a murine dermal wound model.

Main Results:

• PEG architecture and SiP concentration were identified as key factors influencing H2O2 release and adhesive crosslinking.
• The optimized composite adhesive significantly accelerated wound healing compared to controls.
• Minimal epidermal hyperplasia was observed in the treated wounds.

Conclusions:

• The developed catechol-modified bioadhesive effectively promotes wound healing.
• Controlled H2O2 release and soluble silica from SiP contribute to keratinocyte recruitment and accelerated healing.