Rapid determination of p-hydroxylated methamphetamine metabolites by column liquid chromatography-electrochemistry

A method for electrochemical determination of p-hydroxymethamphetamine and p-hydroxyamphetamine in human urine was investigated by column liquid chromatography. A rapid and simple extraction of these substances from urine was performed using a solid-phase extraction column. The detection limit for quantitation was approximately 50 pg for the metabolites. The simplicity and sensitivity of this method allows for analysis of these metabolites in the fields of toxicology and forensic medicine.