How to Build Live-Cell Sensor Microdevices

There is a lot of discussion on how viruses (such as influenza and SARS-CoV-2) are transmitted in air, potentially from aerosols and respiratory droplets, and thus it is important to monitor the environment for the presence of an active pathogen. Currently, the presence of viruses is being determined using primarily nucleic acid-based detection methods, such as reverse transcription- polymerase chain reaction (RT-PCR) tests. Antigen tests have also been developed for this purpose. However, most nucleic acid and antigen methods fail to discriminate between a viable and a non-viable virus. Therefore, we present an alternative, innovative, and disruptive approach involving a live-cell sensor microdevice that captures the viruses (and bacteria) from the air, becomes infected by them, and emits signals for an early warning of the presence of pathogens. This perspective outlines the processes and components required for living sensors to monitor the presence of pathogens in built environments and highlights the opportunity to use immune sentinels in the cells of normal human skin to produce monitors for indoor air pollutants.