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Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis System.

Dong-Yan Tsai1, Chun-Hung Wang1, Perry G Schiro1

  • 1MiCareo Taiwan Co., Ltd., 5F, No. 69, Ln. 77, Xing Ai Rd., Neihu Dist., Taipei City 114, Taiwan.

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Summary
This summary is machine-generated.

Analyzing memory B cells (MBCs) offers crucial insights into SARS-CoV-2 immune escape. A new assay quantifies spike-RBD-specific MBCs, correlating with neutralizing antibodies even months post-vaccination.

Keywords:
SARS-CoV-2memory B cellsrare cellsvaccines

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Area of Science:

  • Immunology
  • Virology
  • Biotechnology

Background:

  • SARS-CoV-2 mutations necessitate advanced immune monitoring beyond neutralizing antibodies.
  • Memory B cells (MBCs) are critical for long-term immunity and require routine analysis.

Purpose of the Study:

  • To develop and validate a novel assay for quantifying antigen-specific MBCs.
  • To correlate MBC levels with neutralizing antibody (nAb) titers post-vaccination.
  • To assess cellular immunity against SARS-CoV-2 variants.

Main Methods:

  • Collected plasma and peripheral blood mononuclear cells (PBMCs) from 35 subjects.
  • Developed a microfluidic chip assay using the MiSelect R II System.
  • Quantified spike-receptor-binding domain (RBD)-specific MBCs in PBMCs.

Main Results:

  • MBC counts strongly correlated with nAb levels, persisting for 6 months post-vaccination.
  • Omicron spike-RBD-specific MBCs were detected after booster vaccination, showing high inter-subject variability.
  • The MiSelect R II System provided a direct, automated, and quantitative method for rare cell analysis.

Conclusions:

  • The MiSelect R II System effectively tracks cellular immunity against SARS-CoV-2.
  • MBC analysis complements nAb studies for comprehensive immune surveillance.
  • This method is vital for understanding immunity against rapidly mutating viruses like SARS-CoV-2.