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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

486
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
486
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

476
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
476

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Related Experiment Video

Updated: Aug 1, 2025

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

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Published on: November 10, 2016

10.5K

Raman spectroscopy for viral diagnostics.

Jijo Lukose1, Ajaya Kumar Barik1, Mithun N1

  • 1Centre of Excellence for Biophotonics, Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, 576104 Manipal, India.

Biophysical Reviews
|April 28, 2023
PubMed
Summary
This summary is machine-generated.

Raman spectroscopy techniques show promise for detecting viruses at very low concentrations. Surface-enhanced Raman scattering, combined with nanotechnology and machine learning, offers efficient and reproducible viral detection, including for SARS-CoV-2.

Keywords:
Point of care applicationsRaman spectroscopyRaman tweezerSARS-CoV-2Surface-enhanced Raman spectroscopyTip-enhanced Raman spectroscopyVirus

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Area of Science:

  • Spectroscopy
  • Biophysics
  • Nanotechnology

Background:

  • Raman spectroscopy enables molecular fingerprinting at ultra-low concentrations.
  • This capability holds significant potential for sensitive virus detection.
  • Accurate and rapid viral diagnostics are crucial for public health.

Purpose of the Study:

  • To review various Raman spectroscopy techniques for virus investigation.
  • To highlight the role of surface-enhanced Raman scattering (SERS) in viral detection.
  • To discuss the application of these methods for SARS-CoV-2 diagnosis.

Main Methods:

  • Review of conventional Raman spectroscopy.
  • Discussion of surface-enhanced Raman spectroscopy (SERS).
  • Exploration of Raman tweezer, tip-enhanced Raman Spectroscopy (TERS), and coherent anti-Stokes Raman scattering (CARS).
  • Integration of nanotechnology, microfluidics, and machine learning with SERS.

Main Results:

  • Various Raman techniques are applicable to virus investigation.
  • SERS, when multiplexed with nanotechnology and microfluidics, enhances spectral reproducibility and workflow efficiency.
  • These techniques demonstrate potential for diagnosing specific viruses like SARS-CoV-2.

Conclusions:

  • Raman spectroscopy is a powerful tool for ultra-low concentration biological molecule detection.
  • SERS offers a promising platform for sensitive and efficient viral detection.
  • The reviewed techniques, particularly SERS, are relevant for diagnosing viral infections such as SARS-CoV-2.