Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Which electrodic solutions for immobilized pH gradients?

E Gianazza, L Quaglia, P Caccia

    Journal of Biochemical and Biophysical Methods
    |April 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Long-Range Transverse-Momentum Correlations and Radial Flow in Pb-Pb Collisions at the LHC.

    Physical review letters·2026
    Same author

    Search for Quasiparticle Scattering in the Quark-Gluon Plasma with Jet Splittings in pp and Pb-Pb Collisions at sqrt[s_{NN}]=5.02  TeV.

    Physical review letters·2025
    Same author

    First Measurement of A=4 Hypernuclei and Antihypernuclei at the LHC.

    Physical review letters·2025
    Same author

    Probing Strangeness Hadronization with Event-by-Event Production of Multistrange Hadrons.

    Physical review letters·2025
    Same author

    Measurements of Chemical Potentials in Pb-Pb Collisions at sqrt[s_{NN}]=5.02  TeV.

    Physical review letters·2024
    Same author

    Observation of Medium-Induced Yield Enhancement and Acoplanarity Broadening of Low-p_{T} Jets from Measurements in pp and Central Pb-Pb Collisions at sqrt[s_{NN}]=5.02  TeV.

    Physical review letters·2024

    Carrier ampholytes are ideal electrodic solutions for immobilized pH gradients (IPGs), efficiently removing ions and accelerating protein migration. This allows direct IPG use after polymerization, eliminating washing steps and improving sample loading.

    Area of Science:

    • Proteomics
    • Biochemistry
    • Analytical Chemistry

    Background:

    • Immobilized pH gradients (IPGs) are crucial for high-resolution protein separation.
    • Traditional IPG methods often require pre-washing steps to remove polymerization catalysts and unreacted monomers.
    • Sample ion interference can lead to conductivity issues and reduced separation efficiency.

    Purpose of the Study:

    • To evaluate carrier ampholytes as superior electrodic solutions for IPGs.
    • To demonstrate the benefits of carrier ampholytes in simplifying IPG protocols and enhancing performance.
    • To investigate the impact of carrier ampholytes on ion removal and protein migration.

    Main Methods:

    • Utilized carrier ampholytes as electrodic solutions for IPGs.
    • Compared ion-binding capacity with acidic/basic amino acid solutions.

    Related Experiment Videos

  • Assessed the necessity of washing steps post-polymerization.
  • Evaluated the effect on sample loading and protein migration speed.

    • Main Results:

    • Carrier ampholytes effectively removed significantly higher amounts of non-buffering ions compared to amino acids.
    • IPGs could be run directly after polymerization without washing when using carrier ampholytes.
    • Eliminated the need for dialysis or larger slab sizes to manage sample ions.
    • Protein migration towards equilibrium was accelerated in the presence of carrier ampholytes (0.3-1% concentration).

    Conclusions:

    • Carrier ampholytes represent a highly suitable electrodic solution for IPGs, simplifying protocols.
    • Their use enhances IPG performance by improving ion management and protein migration.
    • Direct IPG usage post-polymerization is feasible, especially with urea/detergent-containing gels.