Aberrant Expression of FGFRL1 in Esophageal Cancer and Its Regulation by miR-107

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Priyanka Sharma1,2, Vaishali Kaushik1, Anoop Saraya3

  • 1University School of Biotechnology, Guru Gobind Singh Indraprastha University, Dwarka, New Delhi, India.

Asian Pacific Journal of Cancer Prevention : APJCP
|April 28, 2023
PubMed
Abstract

Insights

Fibroblast growth factor receptor like 1 (FGFRL1) is upregulated in esophageal cancer (EC). MiR-107 directly targets and downregulates FGFRL1, indicating a clinical and functional role in EC development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Fibroblast growth factor receptors (FGFRs) are tyrosine kinases crucial for development and cancer.
  • The expression and function of FGFRL1 in esophageal cancer (EC) remain largely uncharacterized.

Purpose of the Study:

  • To investigate the expression and clinical significance of FGFRL1 in esophageal cancer.
  • To identify regulatory mechanisms of FGFRL1 in EC, focusing on microRNA interactions.

Main Methods:

  • Systematic evaluation of FGFRL1 expression in TCGA and GETex datasets.
  • Immunofluorescence and immunohistochemistry for FGFRL1 in EC cell lines and clinical tissues.
  • In-silico prediction, qRT-PCR, and luciferase reporter assays to analyze miR-107 regulation of FGFRL1.

Main Results:

  • FGFRL1 was significantly upregulated in EC tissues and cell lines compared to normal controls.
  • FGFRL1 expression increased with EC progression (WDSCC to PDSCC).
  • MiR-107 was identified as a direct regulator of FGFRL1, inversely correlated with its expression and significantly downregulating it at both mRNA and protein levels.

Conclusions:

  • FGFRL1 plays a significant clinical and functional role in esophageal cancer.
  • MiR-107 directly targets and downregulates FGFRL1, suggesting a novel regulatory axis in EC.
Keywords:
Esophageal squamous cell carcinomaFibroblast growth factor receptorTCGAmiRNA mediated regulation

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