Staphylococcus aureus Colony Polymerase Chain Reaction
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Updated: Aug 1, 2025
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Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
Published on: November 4, 2016
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Merve S Zeden1, Christopher F Schuster2, Angelika Gründling3
1Microbiology, School of Biological and Chemical Sciences, University of Galway, Galway, H91 TK33, Ireland.
Cold Spring Harbor Protocols
|April 28, 2023
Abstract:
Here, we describe a protocol for a colony polymerase chain reaction (PCR) method for Staphylococcus aureus The methodology involves the preparation of small S. aureus lysates by using the enzyme lysostaphin to degrade the peptidoglycan layer. These lysates are prepared using a small patch of bacteria grown on LB agar plates, and the lysates can subsequently be used for PCR analyses.
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