Related Experiment Video
Updated: Aug 1, 2025
14:18
Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
Published on: February 13, 2012
21.3K
Neuronal Store-Operated Calcium Channels
1Université Grenoble Alpes, CNRS, CEA, Inserm UA13 BGE, 38000, Grenoble, France. alexandre.bouron@cea.fr.
Molecular Neurobiology
|April 28, 2023
Summary
Store-operated calcium entry (SOCE) is crucial in non-excitable cells. This review confirms SOCE
Area of Science:
- Neuroscience
- Cell Biology
- Calcium Signaling
Background:
- The endoplasmic reticulum (ER) is a primary intracellular calcium (Ca2+) store in eukaryotic cells.
- Store-operated calcium entry (SOCE) involves Ca2+ uptake via cell surface channels (SOCCs) after ER Ca2+ store depletion.
- SOCE is well-established in non-excitable cells but its presence in neurons has been debated.
Purpose of the Study:
- To review evidence supporting depletion-dependent Ca2+ entry in neurons.
- To discuss the molecular identity, expression, and properties of neuronal SOCCs.
- To explore the physiological significance of SOCE in neuronal function.
Main Methods:
- Review of experimental studies on neuronal calcium signaling.
- Analysis of data on ER Ca2+ store depletion and subsequent Ca2+ influx.
- Examination of molecular, pharmacological, and functional evidence for neuronal SOCCs.
Main Results:
- Growing evidence supports the existence of SOCE in neurons.
- Neuronal SOCCs are recruited following ER Ca2+ store depletion.
- Studies indicate specific molecular compositions and physiological roles for neuronal SOCE.
Conclusions:
- Neuronal SOCE is a significant Ca2+ influx pathway.
- Understanding neuronal SOCCs is vital for comprehending neuronal physiology.
- This pathway represents a key target for future research and therapeutic interventions.
Related Concept Videos
01:19The Role of Ion Channels in Neuronal Computation
3.3K
A postsynaptic neuron usually receives numerous impulses from several other presynaptic neurons. The axon hillock of the postsynaptic neuron integrates all these signals and determines the likelihood of firing an action potential.
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential....
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential....
3.3K
01:30Ligand-Gated Ion Channel Receptor: Gating Mechanism
2.3K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
2.3K
01:26Voltage-gated Ion Channels
8.4K
Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several...
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several...
8.4K
01:24Non-gated Ion Channels
6.9K
Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism....
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism....
6.9K
01:27Feedback Regulation of Calcium Concentration
3.4K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.4K
01:12Mechanically-gated Ion Channels
6.5K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
6.5K