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A decrease in GTP content is associated with aerial mycelium formation in Streptomyces MA406-A-1.

K Ochi

    Journal of General Microbiology
    |February 1, 1986
    PubMed
    Summary
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    Excess nutrients suppress aerial mycelium formation in Streptomyces. Decoyinine, a GMP synthetase inhibitor, triggers aerial mycelium by reducing GTP pools, but does not increase antibiotic production.

    Area of Science:

    • Microbiology
    • Biochemistry
    • Molecular Biology

    Background:

    • Aerial mycelium formation is a key developmental process in Streptomyces.
    • Nutrient availability influences Streptomyces development and antibiotic production.
    • The precise molecular signals regulating aerial mycelium formation are not fully understood.

    Purpose of the Study:

    • To investigate the role of intracellular guanine nucleotide pools in aerial mycelium formation in Streptomyces.
    • To determine the effect of GMP synthetase inhibition on Streptomyces development and antibiotic biosynthesis.
    • To elucidate the signaling pathways involved in Streptomyces aerial mycelium development.

    Main Methods:

    • Culturing Streptomyces sp. MA406-A-1 and other Streptomyces species under nutrient-rich and nutrient-limited conditions.

    Related Experiment Videos

  • Treating cultures with decoyinine, a specific inhibitor of GMP synthetase.
  • Measuring intracellular guanosine triphosphate (GTP) pools.
  • Assessing aerial mycelium formation and antibiotic production (formycin, actinomycin D).

    • Main Results:

    • Excess nutrients suppressed aerial mycelium formation in Streptomyces.
    • Decoyinine initiated aerial mycelium formation in suppressed cultures at concentrations that partially inhibited growth.
    • Decoyinine treatment led to a marked decrease in intracellular GTP pools.
    • Decoyinine initiated aerial mycelium formation in multiple Streptomyces species.
    • Antibiotic production (formycin, actinomycin D) decreased upon decoyinine addition, irrespective of aerial mycelium formation.

    Conclusions:

    • Aerial mycelium formation in Streptomyces is triggered by a decrease in the intracellular guanosine triphosphate (GTP) or guanosine diphosphate (GDP) pool.
    • Antibiotic synthesis in Streptomyces is regulated by distinct signaling pathways, separate from those controlling aerial mycelium formation.
    • Targeting GMP synthetase offers a potential strategy to manipulate Streptomyces development, but not necessarily to enhance antibiotic yields.