Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

14.4K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
14.4K
Complexation Equilibria: The Chelate Effect01:19

Complexation Equilibria: The Chelate Effect

577
In complexation reactions, metal atoms or cations interact with ligands to form donor-acceptor adducts called metal complexes. Ligands that bind through one donor site are monodentate, ligands with two donor sites are bidentate, and those with more than two donor sites are polydentate ligands. For example, ethylene diamine is a bidentate ligand that binds through two nitrogen donor atoms, forming a five-membered ring. EDTA is a polydentate ligand that binds through four oxygen and two nitrogen...
577
Complexometric Titration: Ligands00:43

Complexometric Titration: Ligands

1.0K
Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...
1.0K
Metal-Ligand Bonds02:51

Metal-Ligand Bonds

21.2K
The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
21.2K
EDTA: Chemistry and Properties01:22

EDTA: Chemistry and Properties

2.0K
Polydentate ligands are most widely used in complexometric titrations because they form more stable complexes with the metal ions than mono- or bidentate ligands due to the chelate effect. Examples of polydentate ligands are ethylenediaminetetraacetic acid (EDTA), crown ethers, and cryptands. The most important feature of optimal polydentate ligands is the ability to form 1:1 complexes in a single-step process. Amino carboxylic acid derivatives are frequently used as complexing agents. EDTA is...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Platinum-based phosphorescent lifetime probes for the visualisation of G-quadruplex DNA in cells.

Chemical science·2026
Same author

Visualisation of adjuvant penetration into plant waxes by fluorescence of Nile Red.

Journal of materials chemistry. B·2026
Same author

Affordable, cleanroom-free millifluidic production of targeted lipid nanocarriers <i>via</i> additive manufacturing.

Lab on a chip·2026
Same author

Assembling Lipid Membrane Scaffolds on Microgel-Based Artificial Cells through Vesicle Fusion onto the Hydrogel Network.

ACS nano·2026
Same author

Correction to "Greening the Solid-Phase Peptide Synthesis of the First Bicyclic Analogue of the Arc Repressor and Its Binding to DNA".

The Journal of organic chemistry·2025
Same author

High-affinity A/T-rich DNA binding with a dimeric bisbenzamidine.

NAR molecular medicine·2025
Same journal

Neutral Amphiphiles Boost Transfection Efficiency and Reduce Inflammation in Polymer Micelle-Mediated mRNA Delivery.

Bioconjugate chemistry·2026
Same journal

Surfactant-Mediated Buchwald-Hartwig Coupling of Aliphatic Amines for the Synthesis of DNA-Encoded Libraries.

Bioconjugate chemistry·2026
Same journal

Artificial Intelligence for Discovery in Life Sciences.

Bioconjugate chemistry·2026
Same journal

Iron Single Atom Nanozyme-Mediated GPX4 Inhibitor Delivery for Self-Enhanced Ferroptosis.

Bioconjugate chemistry·2026
Same journal

SpyCatcher-Engineered Ferritin Nanocages Enable Dual-Receptor Targeting for Enhanced Glioma Therapy.

Bioconjugate chemistry·2026
Same journal

One-Pot Synthesis of Functionalized Coumarin Fluorophores Enables Rapid Access to Live-Cell Bioorthogonal Labeling and Microenvironmental Sensing Agents.

Bioconjugate chemistry·2026
See all related articles

Related Experiment Video

Updated: Aug 1, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

773

Dimeric Metal-Salphen Complexes Which Target Multimeric G-Quadruplex DNA.

Timothy Kench1, Viktoria Rakers1, David Bouzada2

  • 1Department of Chemistry, Imperial College London, White City Campus, 82 Wood Lane, London W12 0BZ, United Kingdom.

Bioconjugate Chemistry
|April 29, 2023
PubMed
Summary
This summary is machine-generated.

Dimeric metal-salphen complexes show high selectivity for dimeric G-quadruplex DNA structures over monomeric ones. These complexes offer potential for targeted drug development and studying G-quadruplex interactions.

More Related Videos

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.0K
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

9.6K

Related Experiment Videos

Last Updated: Aug 1, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

773
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.0K
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

9.6K

Area of Science:

  • Medicinal Chemistry
  • Biochemistry
  • Molecular Biology

Background:

  • G-quadruplex (G4) DNA structures are crucial in biological processes and are promising drug targets.
  • Genomic sequences can form both monomeric and multimeric G4s, with human telomeres exhibiting adjacent G4 formation.

Purpose of the Study:

  • To synthesize dimeric metal-salphen complexes using click chemistry.
  • To investigate the selectivity of these dimeric complexes for dimeric versus monomeric G-quadruplexes.
  • To explore the utility of platinum(II)-salphen dimeric complexes in studying G4 interactions and cellular behavior.

Main Methods:

  • Modular synthesis of dimeric metal-salphen complexes (NiII, PtII) via click chemistry.
  • Circular dichroism (CD) spectroscopy to assess G4 binding selectivity.
  • Utilizing emissive properties of PtII complexes for in vitro interaction studies and cellular uptake analysis.

Main Results:

  • Synthesized dimeric metal-salphen complexes with polyether or peptide linkers.
  • CD spectroscopy revealed higher selectivity for dimeric G-quadruplexes compared to monomeric ones.
  • PtII-salphen dimeric complexes demonstrated utility in studying G4 interactions and cellular localization.

Conclusions:

  • Dimeric metal-salphen complexes exhibit enhanced selectivity for dimeric G-quadruplexes.
  • These complexes serve as valuable tools for investigating G-quadruplex biology and developing G4-targeting therapeutics.