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Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...

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Related Experiment Video

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Detection of Bacteria Using Fluorogenic DNAzymes
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Controlled E. coli Aggregation Mediated by DNA and XNA Hybridization.

Cécile Gasse1, Puneet Srivastava2, Guy Schepers2

  • 1Génomique Métabolique, Genoscope Institut François Jacob, CEA, CNRS Univ Evry, Université Paris-Saclay, 2 Rue Gaston Crémieux, 91057, Evry, France.

Chembiochem : a European Journal of Chemical Biology
|April 29, 2023
PubMed
Summary

Researchers developed a novel method for bacterial aggregation using xeno nucleic acids (XNA) and SNAP-tag technology. This strategy enables precise control over bacterial clustering for potential applications in synthetic biology and medicine.

Keywords:
XNAbioconjugationcell surface engineeringoligonucleotidessynthetic biology

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Area of Science:

  • Biotechnology and Synthetic Biology
  • Chemical Biology
  • Microbial Engineering

Background:

  • Chemical cell surface modification is crucial for applications in tissue engineering, immunotherapy, and regenerative medicine.
  • Engineering bacterial tissues via chemical modification is underexplored, highlighting a need for new molecular tools.
  • Developing methods for controlled bacterial aggregation is essential for various biotechnological applications.

Purpose of the Study:

  • To develop an orthogonal nucleic acid-protein conjugation strategy for artificial bacterial aggregation.
  • To explore the use of xeno nucleic acids (XNA) for cell surface modification and bacterial clustering.
  • To establish a modular and reversible system for controlling bacterial aggregation.

Main Methods:

  • Immobilization of XNA (1,5-anhydrohexitol nucleic acids) onto bacterial cell surfaces.
  • Utilizing SNAP-tag mediated covalent interactions for stable XNA attachment.
  • Leveraging oligonucleotide hybridization for reversible bacterial aggregation.

Main Results:

  • Successful covalent immobilization of XNA on cell surfaces via SNAP-tag interactions.
  • Demonstration of artificial bacterial aggregation induced by the XNA-protein conjugation strategy.
  • Establishment of a system combining protein tag stability with oligonucleotide modularity for aggregation control.

Conclusions:

  • The developed orthogonal nucleic acid-protein conjugation strategy effectively promotes artificial bacterial aggregation.
  • This novel approach using XNA and SNAP-tag technology provides a versatile tool for bacterial engineering.
  • The findings open new avenues for applications in synthetic biology, microbial consortia design, and targeted drug delivery.