Activity-based bioluminescence probes for in vivo sensing applications

Area of Science:

• Biomedical imaging
• Molecular imaging
• In vivo imaging

Background:

• Bioluminescence imaging is a sensitive technique for in vivo studies.
• Activity-based sensing (ABS) probes are emerging tools for bioluminescence imaging.
• These probes utilize caged luciferin analogs to detect specific biomarkers.

Purpose of the Study:

• To review recent advancements in bioluminescence-based ABS probes (2021-2023).
• To emphasize the design principles and in vivo validation of these probes.
• To highlight the potential of ABS probes in studying health and disease models.

Main Methods:

• Literature review of recent (2021-2023) bioluminescence ABS probe development.
• Analysis of probe design strategies, focusing on luciferin analog 'caging'.
• Evaluation of in vivo validation studies demonstrating biomarker detection capabilities.

Main Results:

• Several novel bioluminescence ABS probes have been developed.
• These probes demonstrate selective detection of various biomarkers in vivo.
• The design strategies focus on controlled release of luciferin analogs upon biomarker interaction.

Conclusions:

• Bioluminescence-based ABS probes represent a significant advancement in molecular imaging.
• These probes enhance the capability to study biological processes and disease states in vivo.
• Continued development promises broader applications in preclinical research and diagnostics.