Substrate use and temperature effects in flight muscle mitochondria from an endothermic insect, the hawkmoth Manduca...

Manduca sexta are endothermic insects, requiring adult thorax temperatures to be elevated above 35 °C for flight muscles to produce the wing beat frequencies necessary for flight. During flight, these animals rely on aerobic production of ATP by flight muscle mitochondria with several potential metabolic pathways providing the fuel. Along with typical carbohydrate substrates, mitochondria of other endothermic insects including bumblebees and wasps can use the amino acid proline or glycerol 3-phosphate (G3P) as metabolic fuel for prewarm up and flight. Here we examine flight muscle mitochondria physiology and the role of temperature and substrates in oxidative phosphorylation from 3-day old adult Manduca sexta. Mitochondria oxygen flux from flight muscle fibers were temperature sensitive with Q₁₀ values ranging from 1.99 to 2.90, with a large increase in LEAK respiration with increased temperature. Mitochondria oxygen flux was stimulated by carbohydrate-based substrates, with flux through Complex I substrates providing the greatest oxygen flux. Neither proline nor G3P produced an increase in oxygen flux of the flight muscle mitochondria. Unlike other endothermic insects, Manduca are unable to supplement carbohydrate oxidation with either proline or G3P entering through Coenzyme Q and rely on substrates entering at complex I and II.