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Low serum Klotho reflects senile inflammation in middle-aged and elderly patients with coronary atherosclerosis
Qi Mao1, Mengyang Deng1, Jianhua Zhao1
1Department of Cardiology, Institute of Cardiovascular Research, Xinqiao Hospital, Army Medical University, Chongqing 400037, China.
Insights
Low serum Klotho levels indicate aging-related inflammation and are linked to the severity of coronary artery disease (CAD) in older adults. This finding highlights Klotho
Area of Science:
- Gerontology
- Immunology
- Cardiology
Background:
- Atherosclerosis is a chronic inflammatory disease.
- The anti-aging protein Klotho's role in inflammation, particularly senile inflammation, remains unclear.
- This study investigates Klotho's association with inflammation in elderly patients with coronary artery disease (CAD).
Purpose of the Study:
- To ascertain the correlation of serum Klotho with inflammation markers in middle-aged and elderly patients with CAD.
- To evaluate Klotho's relationship with circulating inflammatory markers and T-lymphocyte subsets.
- To determine the predictive value of Klotho and inflammatory markers for significant CAD.
Main Methods:
- Included 302 patients with confirmed CAD.
- Measured serum Klotho, IL-6, and IL-8 concentrations.
- Analyzed T-lymphocyte subsets (monocytes, CD4+/CD8+ ratio) via flow cytometry.
Main Results:
- Serum Klotho independently predicted lower monocyte counts and CD4+/CD8+ ratio.
- Klotho was independently associated with lower IL-6 and IL-8 levels.
- Monocytes and CD4+/CD8+ ratio correlated with higher IL-6 and IL-8, independent of CRP.
- IL-6, IL-8, and CD4+/CD8+ ratio showed excellent predictive performance for significant CAD.
Conclusions:
- Decreased serum Klotho concentrations reflect senile inflammation.
- Low Klotho levels are associated with the severity of CAD in middle-aged and elderly individuals.
- Klotho may serve as a potential biomarker for inflammation and CAD severity.
Background:
Anti-aging protein Klotho has been reported to be associated with atherosclerosis, which was considered as a chronic inflammatory disease. However, the relationship between Klotho and senile inflammation remained unclear. The present study aims to ascertain the correlation of Klotho with inflammation in middle-aged and elderly coronary atherosclerotic disease (CAD).
Methods:
A total of 302 patients with CAD were included in this study. Coronary atherosclerosis was confirmed and quantified for all patients by coronary angiography. Serum Klotho was detected by enzyme linked immunosorbent assay. Serum concentrations of IL-6 and IL-8 were quantified by chemiluminescence assay. T-lymphocyte subsets were measured using flow cytometry.
Results:
Multivariate linear regression analysis showed that serum Klotho was an independent predictor for circulating monocytes (standard β = -0.321, P < 0.001) and CD4+/CD8+ ratio (standard β = -0.522, P < 0.001). After adjustment, serum Klotho was still independently associated with IL-6 (standard β = -0.395, P < 0.001) and IL-8 (standard β = -0.296, P < 0.001). Moreover, circulating monocytes, CD4+ and CD8+ lymphocytes were correlated with increased serum concentrations of IL-6 and IL-8, independent of CRP (P < 0.05). In receiver operating characteristic curve analysis, CD4+/CD8+ ratio (AUC = 0.863, P < 0.001), IL-6 (AUC = 0.893, P < 0.001) and IL-8 (AUC = 0.884, P < 0.001) presented the excellent predictive performance for significant CAD.
Conclusions:
Decreased concentrations in serum Klotho reflect senile inflammation, which is related to the severity of CAD in middle-aged and elderly patients.
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