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Updated: Aug 1, 2025
11:48
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
Published on: October 9, 2014
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Identification of a comprehensive alternative splicing function during epithelial-mesenchymal transition
Yushan Qiu1, Yahong Zhang2, Liwen Tian2
1College of Mathematics and Statistics, Shenzhen University, Shenzhen 518060, P.R.China.
Iscience
|May 1, 2023
Summary
Alternative splicing (AS) plays a key role in tumor metastasis by regulating epithelial-to-mesenchymal transition (EMT). This study identifies significant AS events linked to EMT, revealing their importance in cancer progression.
Area of Science:
- Oncology
- Molecular Biology
- Bioinformatics
Background:
- Epithelial-to-mesenchymal transition (EMT) drives tumor metastasis, but its regulation by alternative splicing (AS) is understudied.
- Transcriptional regulation of EMT is well-understood, yet post-transcriptional mechanisms like AS remain largely uncharacterized.
Purpose of the Study:
- To investigate the role of alternative splicing (AS) in regulating epithelial-to-mesenchymal transition (EMT).
- To develop computational methods for identifying significant AS events associated with EMT using RNA-seq data.
Main Methods:
- Utilized regularization models to identify significant alternative splicing (AS) events during EMT.
- Analyzed RNA-sequencing datasets to associate mRNA isoform variations with EMT.
Main Results:
- Identified key AS events critical for EMT processes.
- Predicted AS events were experimentally validated to be closely related to apoptosis, focal adhesion-invadopodium shift, and tight junction formation.
Conclusions:
- Highlights the significant role of post-transcriptional regulation, specifically AS, in EMT.
- Identified specific AS events as crucial regulatory nodes in tumor metastasis and EMT.
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