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Related Concept Videos

CRISPR01:59

CRISPR

52.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.1K

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Updated: Aug 1, 2025

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
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CRISPR screening in cardiovascular research.

Haihuan Shan1,2, Teng Fei1,2

  • 1National Frontiers Science Center for Industrial Intelligence and Systems Optimization, Key Laboratory of Bioresource Research and Development of Liaoning Province, College of Life and Health Sciences, Northeastern University, Shenyang, China.

Frontiers in Cell and Developmental Biology
|May 1, 2023
PubMed
Summary
This summary is machine-generated.

CRISPR screening, a powerful genome editing tool, is revolutionizing functional genomics. This review explores its application in cardiovascular and metabolic research, highlighting progress and future potential.

Keywords:
CRISPRcardiovasculardiseasescreenscreening

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Area of Science:

  • Biomedical Research
  • Functional Genomics
  • Cardiovascular and Metabolic Disorders

Background:

  • CRISPR-based genome editing tools have significantly advanced biomedical research.
  • CRISPR screening offers high perturbation efficiency and scalability for large-scale genetic studies.
  • While successful in cancer research, CRISPR screening applications in other fields are less explored.

Purpose of the Study:

  • To review the implementation of CRISPR screening in cardiovascular research.
  • To highlight scientific advancements achieved using CRISPR screening in this domain.
  • To envision the future potential of CRISPR screening for cardiovascular and metabolic discoveries.

Main Methods:

  • Review of existing literature on CRISPR screening in cardiovascular and metabolic research.
  • Analysis of scientific progress and challenges.
  • Discussion of future directions and potential applications.

Main Results:

  • CRISPR screening has been applied to investigate genetic factors in cardiovascular and metabolic disorders.
  • Significant, though less extensive than in cancer, progress has been made.
  • The technique holds promise for accelerating discoveries in these complex disease areas.

Conclusions:

  • CRISPR screening is a valuable tool for functional genomics in cardiovascular and metabolic research.
  • Further exploration and application of CRISPR screening are needed to fully realize its potential.
  • This technology can expedite the understanding and treatment of related disorders.