Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A rapid method for monocyte isolation and chemotaxis.

R M Antrum, J S Solomkin

    Journal of Clinical & Laboratory Immunology
    |March 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Meta-analysis and trial sequential analysis of triclosan-coated sutures for the prevention of surgical-site infection.

    The British journal of surgery·2017
    Same author

    Meta-analysis of lower perioperative blood glucose target levels for reduction of surgical-site infection.

    The British journal of surgery·2016
    Same author

    Mechanisms of human neutrophil oxidant production after severe injury.

    Surgery·2001
    Same author

    Granulocyte-colony stimulating factor in the prevention of postoperative infectious complications and sub-optimal recovery from operation in patients with colorectal cancer and increased preoperative risk (ASA 3 and 4). Protocol of a controlled clinical trial developed by consensus of an international study group. Part one: rationale and hypothesis.

    Inflammation research : official journal of the European Histamine Research Society ... [et al.]·2001
    Same author

    Antibiotic resistance in postoperative infections.

    Critical care medicine·2001
    Same author

    Focus on "Essential role for IL-6 in postresuscitation inflammation in hemorrhagic shock".

    American journal of physiology. Cell physiology·2001
    Same journal

    Policy and science of FMD control: the stakeholders' contribution to decision making. A call for integrated animal disease management.

    Journal of clinical & laboratory immunology·2006
    Same journal

    An informative case of Graves' disease with implications for schizophrenia.

    Journal of clinical & laboratory immunology·2006
    Same journal

    Increase of circulating CD8+CD57+ lymphocytes after measles infection but not after measles vaccination.

    Journal of clinical & laboratory immunology·2006
    Same journal

    Review: immunomodulatory activity of pregnancy-associated plasma protein-A.

    Journal of clinical & laboratory immunology·2004
    Same journal

    Antibodies to Acinetobacter bacteria and bovine brain peptides, measured in bovine spongiform encephalopathy (BSE) in an attempt to develop an ante-mortem test.

    Journal of clinical & laboratory immunology·2004
    Same journal

    Principles of autoimmune disease: pathogenesis, genetics and specific immunotherapy.

    Journal of clinical & laboratory immunology·2004
    See all related articles

    This study presents a rapid 4-hour method for isolating human monocytes and assessing their chemotaxis. The technique uses small blood volumes and yields high-purity monocytes, aiding patient monocyte function studies.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Monocyte isolation and chemotaxis studies are crucial for understanding immune responses.
    • Existing methods can be time-consuming and require large blood volumes.

    Purpose of the Study:

    • To develop a rapid and efficient method for isolating human peripheral blood monocytes.
    • To establish a protocol for studying monocyte chemotaxis using the isolated cells.

    Main Methods:

    • Isolation of monocytes using Dextran T500 sedimentation and Percoll gradient centrifugation.
    • Chemotaxis assays performed in a 48-well chamber with chemoattractants like complement component 5a (C5a) and N-formylmethionyl-leucyl-phenylalanine (FMLP).
    • Checkerboard analysis used to validate chemotactic responses.

    Main Results:

    Related Experiment Videos

    • Achieved a mean monocyte purity of 60% (+/- 10%).
    • Identified that pooled serum activation on PVP-coated filters generates C5a, a potent chemoattractant.
    • Demonstrated that high concentrations of C5a induce chemotaxis comparable to zymosan-activated serum (ZAS).

    Conclusions:

    • The described technique provides a fast (approx. 4 hours) and effective method for monocyte isolation and chemotaxis assessment.
    • Requires only small blood volumes (15 ml), making it suitable for clinical applications.
    • Facilitates sequential investigation of monocyte function in patient samples.