Discrepancies in Lipemia Interference Between Endogenous Lipemic Samples and Smoflipid^®-Supplemented Samples

Area of Science:

• Clinical Chemistry
• Biochemical Analysis
• Diagnostic Interference Studies

Background:

• Intralipid^®, commonly used for lipemia interference studies, lacks diverse lipoprotein types.
• Endogenous lipemic samples and SMOFlipid^®-supplemented samples present different interference patterns.

Purpose of the Study:

• To evaluate lipemia-induced interference in biochemical parameters using both endogenous lipemic samples and SMOFlipid^®-supplemented samples.
• To determine the suitability of SMOFlipid^® for lipemic interference studies.

Main Methods:

• Serum pools were supplemented with SMOFlipid^® to triglyceride concentrations of 800 mg/dL and 1500 mg/dL.
• Lipemic serum pools from patients (800 mg/dL and 1500 mg/dL triglyceride) were ultracentrifuged to remove lipids.
• 25 biochemical parameters were analyzed before and after supplementation/ultracentrifugation; bias was compared.

Main Results:

• At 800 mg/dL, total protein and transferrin were affected only in endogenous lipemic samples; magnesium and creatinine only in SMOFlipid^®-supplemented samples.
• At 1500 mg/dL, total protein, amylase, ferritin, and glucose were affected only in endogenous lipemic samples; chloride only in SMOFlipid^®-supplemented samples.
• Significant differences in interference patterns were observed between the two sample types.

Conclusions:

• SMOFlipid^®-supplemented samples do not accurately reflect lipemia-induced interference.
• Interference studies require diverse endogenous lipemic patient samples to account for lipoprotein heterogeneity.