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A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates
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Phylogenetic relationship of Fusarium species isolated from keratitis using TEF1 and RPB2 gene sequences

Zeynab Yassin1, Zahra Salehi2, Mohammad Soleimani3

  • 1Antimicrobial Resistance Research Center, Iran University of Medical Sciences, Tehran, Iran.

Iranian Journal of Microbiology
|May 1, 2023
PubMed
Abstract

Insights

This study identified Fusarium species causing keratitis using TEF1 and RPB2 gene sequencing. The research differentiated Fusarium solani species complex, Fusarium fujikuroi species complex, and Fusarium oxysporum, aiding in understanding fungal infections.

Area of Science:

  • Medical Mycology
  • Molecular Biology
  • Ophthalmology

Background:

  • * Fusarium* species are a leading cause of fungal keratitis.
  • Accurate identification is crucial due to antifungal resistance.

Purpose of the Study:

  • To identify *Fusarium* species responsible for keratitis.
  • To analyze the genetic diversity of these isolates.
  • To evaluate the efficacy of combined *TEF1* and *RPB2* gene sequencing for identification.

Main Methods:

  • Isolation of 24 *Fusarium* clinical strains from keratitis patients.
  • Phylogenetic analysis using maximum parsimony and RAxML methods.
  • Sequencing of *TEF1* and *RPB2* genes, including novel primer design for *RPB2*.

Main Results:

  • 17 isolates identified as *Fusarium solani* species complex (FSSC).
  • 4 isolates identified as *Fusarium fujikuroi* species complex (FFSC), including *F. proliferatum*, *F. globosum*, *F. verticillioides*, and *F. brevicatenulatum*.
  • 3 isolates identified as *Fusarium oxysporum*.

Conclusions:

  • Combined *TEF1* and *RPB2* gene sequencing effectively differentiates *Fusarium* species causing keratitis.
  • This molecular approach is significant for accurate diagnosis and management of fungal keratitis.
  • Novel primers for *RPB2* enhance identification capabilities.
Keywords:
Fusarium fujikuroi species complexFusarium oxysporumFusarium solani species complexKeratitis

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