Silicon Nanoparticle-Based Ratiometric Fluorescence Probes for Highly Sensitive and Visual Detection of VB₂

In this work, blue fluorescent silicon nanoparticles (SiNPs) were prepared by a simple one-step hydrothermal method using (3-aminopropyl) triethoxy silane (APTES) and eriochrome black T as raw materials. The SiNPs showed favorable water solubility, thermal stability, pH stability, salt tolerance, and photobleaching resistance. At an excitation wavelength of 376 nm, the SiNPs emitted bright blue fluorescence at 460 nm. In the presence of vitamin B₂ (VB₂), the fluorescence intensity (FL intensity) of the SiNPs at 460 nm decreased obviously, and a new peak appeared at 521 nm. Based on this, a novel ratiometric fluorescence method was established for VB₂ detection. There was a good linear relationship between the fluorescence intensity ratio (F ₅₂₁/F ₄₆₀) and VB₂ concentration from 0.5 to 60 μM with a detection limit of 135 nM. This method was successfully applied to detect VB₂ content in the samples of vitamin B₂ drugs and beverages. Additionally, a simple paper sensor based on the SiNPs was designed to visualize detection of VB₂. With the support of color recognition software on a smartphone, the visual quantitative analysis of VB₂ was realized, ranging from 40 to 800 μM.