High-quality manipulable fiber-microsphere for super-resolution microscopy

Despite the gain in resolution brought by microsphere (MS)-assisted microscopy, it has always faced several limitations, such as a limited field of view, surface defects, low contrast, and lack of manipulability. This Letter presents a new type of MS created at the tip of an optical fiber, which we call a fiber microsphere (fMS). The fMS is made from a single-mode or coreless fiber, molten and stretched, ensuring high homogeneity and a sphere diameter smaller than the fiber itself. In addition, the connection between the fMS and the fiber makes scanning the sample a simple task, offering a solution to the difficulties of handling. The fabrication procedure of the fMS and the optical system used in the study are detailed. Our measurements show a clear superiority of the fMS over the soda-lime MS in resolving power and imaging performance.