Analysis of the Adsorbed Vaccine Formulations Using Water Proton Nuclear Magnetic Resonance-Comparison with Optical...

Area of Science:

• Analytical Chemistry
• Vaccine Technology
• Materials Science

Background:

• Aluminum-adjuvanted vaccines are critical for public health.
• Characterizing these complex formulations noninvasively is challenging.
• Accurate analysis ensures vaccine efficacy and safety.

Purpose of the Study:

• To assess water proton nuclear magnetic resonance (wNMR) for aluminum-adjuvanted vaccine analysis.
• To compare wNMR with optical techniques for formulation characterization.
• To evaluate wNMR's noninvasive capabilities on sealed vaccine vials.

Main Methods:

• wNMR and optical methods (laser diffraction, laser scattering) were employed.
• Vaccine formulations with varying antigen loads on AlPO4 microparticles were analyzed.
• Measurements covered dispersed states and sedimentation processes in sealed vials.

Main Results:

• wNMR distinguished high- from low-antigen dose formulations, outperforming laser diffraction.
• wNMR and laser scattering showed comparable results for sedimentation kinetics.
• wNMR provided insights into vaccine compactness and antigen-adjuvant interactions.

Conclusions:

• wNMR is suitable for analyzing aluminum-adjuvanted vaccine formulations.
• wNMR can differentiate vaccines based on antigen load.
• wNMR offers a noninvasive method for inspecting finished vaccine products and antigen-adjuvant complexes.