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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: Jul 31, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

Single-cell analysis targeting the proteome.

Mahmoud Labib¹, Shana O Kelley^2,3,4,5

¹Department of Pharmaceutical Sciences, University of Toronto, Toronto, ON, Canada.

Nature Reviews. Chemistry

|May 2, 2023

Summary

This summary is machine-generated.

Analyzing single cells is crucial for understanding biology. New single-cell protein analysis technologies offer powerful insights into cell and disease biology, despite current challenges.

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Last Updated: Jul 31, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

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Published on: December 9, 2022

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Published on: June 21, 2019

Area of Science:

Biochemistry
Molecular Biology
Cell Biology

Background:

Cellular heterogeneity is fundamental to biological processes.
Single-cell genomics and transcriptomics are established, but proteomic analysis remains difficult.
Advancements in single-cell protein analysis are crucial for biological and medical research.

Purpose of the Study:

To review current single-cell protein analysis technologies.
To assess the advantages and limitations of these emerging methods.
To highlight their potential impact on various research areas.

Main Methods:

Review of existing literature on single-cell protein analysis techniques.
Comparative assessment of different technologies based on their capabilities and drawbacks.
Discussion of the applications and future directions of single-cell proteomic analysis.

Main Results:

Single-cell protein analysis tools are rapidly evolving.
These technologies provide valuable insights into protein expression at the single-cell level.
Various methods offer different strengths and weaknesses for comprehensive proteomic interrogation.

Conclusions:

Emerging single-cell protein analysis technologies hold significant promise.
They can illuminate tumor heterogeneity, therapeutic resistance, and immune responses.
These advancements are expected to accelerate drug discovery and deepen our understanding of cell biology.