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Draft genome sequence data of Enterococcus faecium R9, a multiple enterocins-producing strain
Abrar Akbar1, Sabah Al-Momin1, Mohamed Kishk1
1Environment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, Kuwait.
Data in Brief
|May 2, 2023
Summary
Natural bacteriocins from Enterococcus faecium R9, isolated from camel milk, offer a safer alternative to chemical preservatives. These antimicrobial peptides show promise for controlling Listeria monocytogenes in food products.
Area of Science:
- Microbiology
- Food Science
- Genomics
Background:
- Foodborne pathogens cause significant health issues and economic losses.
- Chemical preservatives raise health concerns, driving the search for natural alternatives.
- Bacteriocins, ribosomally synthesized peptides, are natural antimicrobials with potential for food preservation.
Purpose of the Study:
- To present the draft genome sequence of Enterococcus faecium strain R9.
- To identify bacteriocin-encoding genes within the genome.
- To characterize the technological properties of the produced bacteriocins.
Main Methods:
- Whole-genome sequencing using Illumina HiSeq 2500.
- Bioinformatic analysis for gene prediction and annotation.
- Characterization of bacteriocin properties (proteolytic enzyme sensitivity, heat stability, pH tolerance).
Main Results:
- The genome sequence of Enterococcus faecium R9 comprises 3,598,862 bases with a GC content of 37.94%.
- The genome contains genes encoding for three bacteriocins: enterocin B, enterocin P, and enterocin X (two-component).
- The bacteriocins exhibited desirable technological properties, including heat stability and broad pH tolerance.
Conclusions:
- Enterococcus faecium R9 produces bacteriocins with valuable properties for the food industry.
- These bacteriocins can aid in controlling Listeria monocytogenes contamination.
- The genome sequence provides a foundation for further research and application of these natural preservatives.
