Related Experiment Video
Updated: Jul 31, 2025

The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro
Published on: September 18, 2017
Mesenchymal Stromal Cells Facilitate Tip Cell Fusion Downstream of BMP-Mediated Venous Angiogenesis-Brief Report
Shahram Eisa-Beygi1, Meng-Ming Hu1, Suresh N Kumar2
1Department of Cell Biology, Neurobiology and Anatomy (S.E.-B., M.-M.H., B.E.J., R.F.C., M.B.V., B.A.L.), Medical College of Wisconsin, Milwaukee.
Background:
The goal of this study was to identify and characterize cell-cell interactions that facilitate endothelial tip cell fusion downstream of BMP (bone morphogenic protein)-mediated venous plexus formation.
Methods:
High resolution and time-lapse imaging of transgenic reporter lines and loss-of-function studies were carried out to study the involvement of mesenchymal stromal cells during venous angiogenesis.
Results:
BMP-responsive stromal cells facilitate timely and precise fusion of venous tip cells during developmental angiogenesis.
Conclusions:
Stromal cells are required for anastomosis of venous tip cells in the embryonic caudal hematopoietic tissue.
More Related Videos
08:04In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing
Published on: May 11, 2021
09:24Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells
Published on: September 18, 2019
Related Concept Videos
Regulation of Angiogenesis and Blood Supply
Mechanism of Angiogenesis
Mesenchymal Stem Cells
Overview of Cell-Matrix Interactions