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Colorimetric Sensing Strategy through the Coordination Chemistry between Ascorbic Acid 2-Phosphate and Copper Ions
Yidan Wang1, Yunlei Xianyu1,2,3
1State Key Laboratory of Fluid Power and Mechatronic Systems, College of Biosystems Engineering and Food Science, Zhejiang University, 310058 Hangzhou, China.
A new colorimetric sensing method uses ascorbic acid 2-phosphate (AAP) and copper ions for rapid detection. This approach enables simple, cost-effective monitoring of enzymatic activity, suitable for point-of-care diagnostics.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Materials Science
Background:
- Coordination chemistry of phosphorylated molecules and metal ions is known, but sensing applications are underexplored.
- Ascorbic acid 2-phosphate (AAP) is a phosphorylated molecule with potential for metal ion coordination.
Purpose of the Study:
- To develop a novel colorimetric sensing strategy utilizing the coordination chemistry between AAP and copper ions.
- To establish a method for monitoring enzymatic activity based on AAP-AA transformation and its effect on copper ion coordination.
Main Methods:
- Investigated the coordination reaction between AAP and copper ions, observing a visible blue-to-green color change.
- Quantified the color change using the blue-to-green (B/G) ratio, readable by naked eye or smartphone.
- Utilized alkaline phosphatase to catalyze AAP hydrolysis to ascorbic acid (AA), modulating the colorimetric response.
Main Results:
- A rapid, visible color change from blue to green was observed upon AAP-copper ion coordination.
- The developed colorimetric assay demonstrated good stability, sensitivity, and cost-effectiveness.
- The strategy successfully monitored alkaline phosphatase activity through AAP/AA transformation.
Conclusions:
- The AAP-copper ion coordination chemistry offers a facile and convenient platform for designing colorimetric assays.
- This method shows significant promise for point-of-care diagnostic applications due to its performance and scalability.
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