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Intralipid as a matrix additive for evaluating hyperlipidemic postmortem blood.

Emily Elenstål1, Henrik Green1,2, Robert Kronstrand1,2

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Lipemia significantly interferes with postmortem drug analysis, affecting hydrophilic and lipophilic compounds. Intralipid offers a useful simulation for validating methods in hyperlipidemic samples.

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Area of Science:

  • Forensic Toxicology
  • Analytical Chemistry

Background:

  • Postmortem whole blood quality varies, with hyperlipemia being a common issue affecting analytical results.
  • Accurate quantification of drugs in hyperlipidemic postmortem samples is crucial for forensic investigations.

Purpose of the Study:

  • To investigate the impact of lipemia on postmortem drug analysis.
  • To compare Intralipid and pooled postmortem lipids as matrix additives for simulating hyperlipidemia.
  • To evaluate the method's performance in hyperlipidemic conditions.

Main Methods:

  • Simulated hyperlipidemia in bovine whole blood using Intralipid or pooled postmortem lipids.
  • Spiked samples with 14 benzodiazepines and 5 sedative/antianxiety drugs.
  • Analyzed samples using liquid-liquid extraction and ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS).

Main Results:

  • Lipemia caused significant interference in analyte quantification.
  • Internal standards (ISs) compensated for most analyte losses, except for highly hydrophilic (7-amino metabolites) and lipophilic (propiomazine, dihydropropiomazine) compounds.
  • Intralipid generally resulted in lower analyte recoveries compared to pooled postmortem lipids.

Conclusions:

  • Lipemia poses a major challenge in postmortem drug analysis.
  • Intralipid serves as a valuable tool for simulating lipemic conditions during method development and validation.
  • Careful consideration of matrix effects is necessary for accurate quantification in hyperlipidemic samples.