Related Experiment Video
Updated: Jul 31, 2025
12:51
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
9.0K
Multiplexed Sequential Imaging in Living Cells with Orthogonal Fluorogenic RNA Aptamer/Dye Pairs
Ru Zheng1, Rigumula Wu1, Yuanchang Liu1
1Department of Chemistry, University of Massachusetts, Amherst, MA 01003, USA.
Biorxiv : the Preprint Server for Biology
|May 3, 2023
Summary
Researchers developed sequential Fluorogenic RNA Imaging-Enabled Sensor (seqFRIES) for multiplexed live-cell imaging. This method overcomes spectral overlap challenges, enabling detection of multiple targets within living cells.
Area of Science:
- Cell Biology
- Molecular Imaging
- Biotechnology
Background:
- Multiplexed fluorescence imaging in living cells is crucial but limited by fluorophore spectral overlap.
- Detecting more than two to three targets simultaneously remains a significant technical hurdle.
Conclusions:
- seqFRIES provides a robust method for highly multiplexed and dynamic cellular imaging.
- The validated seqFRIES concept and orthogonal RNA/dye pairs are expected to advance cell biology studies.
- This strategy facilitates broader usage for advanced live-cell imaging applications.
