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Published on: March 30, 2019
MicroRNA-215-5p promotes proliferation, invasion, and inhibits apoptosis in liposarcoma cells by targeting MDM2
Zhengnan Song1, Jingping Bai1, Renbing Jiang1
1Department of Bone and Soft Tissue, Xinjiang Tumor Hospital, Urumqi, China.
Background:
Liposarcoma (LPS) is one of the most common soft tissue malignancies in adults, and it is characterized by dysregulation of multiple signaling pathways, including MDM2 proto-oncogene (MDM2) amplification. MicroRNA (miRNA) regulates gene expression through incomplete complementary pairing with the 3' untranslated region of mRNAs involved in tumor progression.
Methods:
In this study, bioinformatics analysis, RT-qPCR, dual-luciferase reporter gene, MTT, flow cytometry, cell scratches, chamber migration, colony formation, FISH, WB, and CCK8 were used.
Results:
RT-qPCR showed that the expression of MDM2 was increased when miR-215-5p was overexpressed compared with the control group. The dual-luciferase reporter gene showed that the Renilla ratio firefly fluorescence intensity was decreased in the overexpression group compared with the control group. Cell phenotype experiments revealed that the overexpression group had increased cell proliferation rate, increased apoptosis rate, increased colony formation rate, increased cell healing area ratio, and increased number of cell invasions. FISH revealed increased MDM2 expression in the overexpression group. WB suggested decreased Bax expression, increased PCNA, Bcl-2, and MDM2 expression, and decreased P53 and P21 expression in the overexpression group.
Conclusions:
In this study, we suggest that miR-215-5p can target and promote MDM2 expression, promote the proliferation and invasion of LPS cells SW-872, and inhibit apoptosis.Targeting miR-215-5p may be a novel therapeutic strategy for the treatment of LPS.
Insights
MicroRNA-215-5p targets and elevates MDM2 expression, enhancing liposarcoma (LPS) cell proliferation and invasion while inhibiting apoptosis. Targeting this microRNA presents a potential therapeutic strategy for LPS treatment.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Liposarcoma (LPS) is a prevalent soft tissue cancer in adults.
- LPS is characterized by dysregulated signaling pathways, including MDM2 proto-oncogene (MDM2) amplification.
- MicroRNAs (miRNAs) regulate gene expression and are implicated in tumor progression.
Purpose of the Study:
- To investigate the role of miR-215-5p in liposarcoma (LPS).
- To determine the relationship between miR-215-5p and MDM2 expression in LPS cells.
- To explore the potential of targeting miR-215-5p as a therapeutic strategy for LPS.
Main Methods:
- Bioinformatics analysis, RT-qPCR, dual-luciferase reporter assays.
- Cell proliferation (MTT, CCK8), apoptosis (flow cytometry), migration, and colony formation assays.
- Fluorescence in situ hybridization (FISH) and Western blotting (WB) to assess gene and protein expression.
Main Results:
- Overexpression of miR-215-5p increased MDM2 expression in LPS cells.
- miR-215-5p overexpression promoted cell proliferation, invasion, and colony formation.
- Apoptosis was inhibited, while MDM2, PCNA, and Bcl-2 expression increased; Bax, P53, and P21 expression decreased.
Conclusions:
- miR-215-5p targets and promotes MDM2 expression in LPS cells.
- miR-215-5p enhances LPS cell proliferation and invasion while inhibiting apoptosis.
- Targeting miR-215-5p may offer a novel therapeutic approach for liposarcoma.
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