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    Gradient-index (GRIN) rod lens in-line digital holographic microscopy (DHM) offers improved resolution for imaging micro-particles. This compact and cost-effective setup achieves micrometer-scale resolution, outperforming conventional systems.

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    Area of Science:

    • Optics and Photonics
    • Microscopy Techniques
    • Holography

    Background:

    • In-line digital holographic microscopy (DHM) offers 3D imaging with large fields of view and depth of field.
    • Traditional DHM systems often rely on pinholes, which can limit resolution and complicate alignment.
    • Gradient-index (GRIN) rod lenses present an alternative for compact and potentially higher-resolution DHM setups.

    Purpose of the Study:

    • To develop and experimentally demonstrate an in-line DHM system utilizing a GRIN rod lens.
    • To compare the resolution and image quality of the GRIN-based DHM with a conventional pinhole-based DHM.
    • To investigate the theoretical and experimental factors influencing resolution in DHM systems.

    Main Methods:

    • Development of theoretical framework for GRIN rod lens in-line DHM.
    • Experimental implementation of both GRIN-based and pinhole-based in-line DHM configurations.
    • Holographic imaging of polystyrene micro-particles (2.0 and 3.0 µm) using the optimized GRIN setup.
    • Systematic investigation of the effect of source-detector and sample-detector distances on resolution.

    Main Results:

    • The optimized GRIN-based DHM achieved a resolution of approximately 1.38 µm in a high-magnification regime.
    • The GRIN-based setup demonstrated superior resolution compared to the pinhole-based system under specific conditions.
    • Experimental results for resolution and imaging of micro-particles showed good agreement with theoretical predictions.
    • The study successfully imaged dilute polystyrene micro-particles with high fidelity.

    Conclusions:

    • GRIN rod lens in-line DHM is a viable and effective technique for high-resolution 3D imaging.
    • The GRIN-based approach offers advantages in resolution and potentially compactness over pinhole-based DHM.
    • Understanding the impact of optical distances is crucial for optimizing DHM resolution.
    • This technology holds promise for applications requiring detailed micro-particle analysis.