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Protocol to assess fatal embolism risks from human stem cells.

Fei Ma¹, Jinlai Zhang¹, Xin Jin¹

¹Tasly Stem Cell Biology Laboratory, Tianjin 300410, China.

|May 3, 2023

Summary

This study introduces a protocol to identify pro-embolic adipose-derived multipotent stromal cells (ADSCs) and predict fatal embolism risks from ADSC infusions, enhancing cell quality assessment for clinical stem cell applications.

Keywords:

Bioinformatics Health Sciences RNAseq Stem Cells

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Area of Science:

Stem cell biology
Biotechnology
Regenerative medicine

Background:

Adipose-derived multipotent stromal cells (ADSCs) show promise in regenerative medicine.
A critical safety concern for ADSC infusion is the risk of fatal embolism.
Identifying and mitigating this risk is crucial for clinical translation.

Purpose of the Study:

To present a protocol for identifying the pro-embolic sub-population of human ADSCs.
To develop a mathematical model for predicting ADSC embolic risk.
To enhance the assessment of cell quality for clinical stem cell applications.

Main Methods:

Collection, processing, and classification of ADSC single-cell RNA-seq data.
Development of a mathematical model for predicting ADSC embolic risk.
Protocol detailed for ADSC identification and risk prediction.

Main Results:

A protocol to identify pro-embolic ADSC sub-populations has been established.
A mathematical model for predicting ADSC embolic risk has been developed.
The protocol facilitates enhanced cell quality assessment.

Conclusions:

This protocol enables the identification of ADSCs with embolic potential.
The developed mathematical model aids in predicting fatal embolism risks.
Advancing clinical applications of stem cells through improved safety and quality assessment.