Perylene diimide-based sensors for multiple analyte sensing (Fe²⁺/H₂S/ dopamine and Hg²⁺/Fe²⁺): cell imaging and INH,...

In this report, we present our results on the recognition of multiple analytes using trisubstituted PDI-based chemosensors DNP and DNB in 50% HEPES buffered-CH₃CN solution. Upon the addition of Hg²⁺, DNB showed a decrease and increase in absorbance intensity at 560 and 590 nm, respectively, with a detection limit of 7.17 μM and bleaching of the violet color (de-butynoxy). Similarly, the addition of Fe²⁺ or H₂S to the solution of DNP or DNB resulted in ratiometric changes (A_688nm/A_560nm) with respective detection limits of 185 nM and 27.6 nM for Fe²⁺, respectively, and a color change from violet to green. However, the addition of >37 μM H₂S caused a decrease in absorbance at 688 nm with a concomitant blue shift to 634 nm. Upon the addition of dopamine, the DNP + Fe²⁺ assay showed ratiometric (A_560nm/A_688nm) changes within 10 s along with a color change from green to violet. Moreover, DNP has been successfully used for the exogenous detection of Fe²⁺ in A549 cells. Further, the multiple outputs observed with DNP in the presence of H₂S have been used to construct NOR, XOR, INH and 4-to-2 encoder logic gates and circuits.