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Updated: Jul 31, 2026

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization
Published on: December 29, 2013
Recirculating packed-bed biofilm photobioreactor combined with membrane ultrafiltration as advanced wastewater
Oliver Díaz1, Enrique González2, Luisa Vera2
1Departamento de Ingeniería Química y Tecnología Farmacéutica, Facultad de Ciencias, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez s/n, 38206, La Laguna, Spain. odiazlop@ull.edu.es.
Abstract:
Packed-bed biofilm photobioreactor combined with ultrafiltration membrane was investigated for intensifying the process for secondary wastewater effluent treatment. Cylindrical glass carriers were used as supporting material for the microalgal-bacterial biofilm, which developed from indigenous microbial consortium. Glass carriers allowed adequate growth of the biofilm with limited suspended biomass. Stable operation was achieved after a start-up period of 1000 h, where supernatant biopolymer clusters were minimized and complete nitrification was observed. After that time, biomass productivity was 54 ± 18 mg·L-1·day-1. Green microalgae Tetradesmus obliquus and several strains of heterotrophic nitrification-aerobic denitrification bacteria and fungi were identified. Combined process exhibited COD, nitrogen and phosphorus removal rates of 56 ± 5%, 12 ± 2% and 20 ± 6%, respectively. Membrane fouling was mainly caused by biofilm formation, which was not effectively mitigated by air-scouring aided backwashing.
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