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Updated: Jul 31, 2025

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System
Published on: May 22, 2020
A hybrid technique for measurement of intra/extracellular proteins
Haiam Edabashi1, Radwa Elghadafi1, Nischwethaa Rajkanth1
1Department of Periodontology, Henry M. Goldman School of Dental Medicine, Boston University, Boston, MA, United States of America.
Abstract:
ELISA or Western blot is known as a basic technique to be used for measurement of intracellular proteins, but in some cases, they cannot overcome problems such as normalization between samples or extraneous costs for required commercial kits. In order to address this problem, we developed a rapid and effective method (a hybrid of Western blot and ELISA). We use this new hybrid method to detect and normalize trace protein changes in gene expression intracellularly at a lower cost.

