Related Experiment Video
Updated: Jul 31, 2025
08:58
Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats
Published on: August 25, 2020
5.4K
BMP2 induces osteogenic differentiation through ACKR3 in mesenchymal stem cells.
Jiang Liu1, Xin-Tong Yao2, Xiao-Lei Feng3
1Dalian Medical University, Dalian, 116044, Liaoning, China; Department of Orthopedics, The 960th Hospital of the PLA Joint Logistics Support Force, Ji'nan, 250013, Shandong, China.
Summary
ACKR3 protein levels increase with age and inhibit bone formation. Inhibiting ACKR3 enhances bone regeneration, suggesting ACKR3 as a therapeutic target for bone loss diseases.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Orthopedics
Background:
- Bone loss diseases like osteoporosis are increasing.
- Mesenchymal stem cells (MSCs) can differentiate into bone-forming osteoblasts.
- Understanding osteoblast differentiation mechanisms is crucial for treating bone diseases.
Purpose of the Study:
- To investigate the role of ACKR3 in BMP2-induced osteogenic differentiation of MSCs.
- To elucidate the molecular mechanism involving ACKR3/p38/MAPK signaling in bone formation.
- To evaluate ACKR3 as a potential therapeutic target for bone diseases.
Main Methods:
- Measured ACKR3 protein levels in human femoral tissues across different ages and sexes.
- Performed in vitro cellular assays to assess ACKR3's effect on MSC differentiation.
- Conducted in vitro embryo femur culture experiments in C57BL6/J mice.
- Analyzed the involvement of p38/MAPK signaling pathways.
Main Results:
- ACKR3 protein levels increase with age in human femoral tissues.
- ACKR3 inhibits BMP2-induced osteogenic differentiation and promotes adipogenesis in MSCs.
- Inhibition of ACKR3 enhances BMP2-induced trabecular bone formation in mouse models.
- ACKR3 agonist TC14012 suppressed p38 and STAT3 phosphorylation in BMP2-induced MSC differentiation.
Conclusions:
- ACKR3 plays an inhibitory role in BMP2-induced osteogenic differentiation of MSCs.
- The p38/MAPK signaling pathway is implicated in ACKR3's mechanism of action.
- ACKR3 represents a potential novel therapeutic target for bone-associated diseases and bone tissue engineering.
