Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

52.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.5K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

80
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
80
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spore inoculum size impacts substrate degradation and sporulation but not the secretome during colonization of whole yellow pea (Pisum sativum) by Aspergillus oryzae.

International journal of food microbiology·2026
Same author

Cellular dynamics in liquid static cultures of Aureobasidiumpullulans.

Fungal biology·2026
Same author

Functions of the cell wall polysaccharide schizophyllan during vegetative growth of <i>Schizophyllum commune</i>.

Cell surface (Amsterdam, Netherlands)·2026
Same author

Mechanical properties and water absorption of shiitake mushroom-based films with natural rubber latex.

New biotechnology·2025
Same author

Strongest untreated mycelium materials produced by Schizophyllum commune dikaryons.

World journal of microbiology & biotechnology·2025
Same author

Protoplasting of Aspergillus niger using Trichoderma harzianum culture medium after growth on cell walls of the target fungus.

Journal of microbiological methods·2025

Related Experiment Video

Updated: Jul 31, 2025

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
09:56

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Published on: November 14, 2018

12.0K

Inheritable CRISPR based epigenetic modification in a fungus.

Xiaoyi Chen1, Juan P Moran Torres1, Yiling Li1

  • 1Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands.

Microbiological Research
|May 4, 2023
PubMed
Summary

CRISPRoff and CRISPRon systems enable programmable gene silencing and activation in Aspergillus niger. These epigenetic tools offer stable gene control for studying fungal gene function.

Keywords:
AspergillusEpigenetic modificationFungusSpore production

More Related Videos

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

1.2K
CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
07:25

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

9.6K

Related Experiment Videos

Last Updated: Jul 31, 2025

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
09:56

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Published on: November 14, 2018

12.0K
CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

1.2K
CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
07:25

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

9.6K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Mycology

Background:

  • CRISPRoff and CRISPRon are programmable epigenetic editing tools.
  • These systems utilize deactivated Cas9 (dCas9) fused to effector domains.
  • Previous applications were limited to human cells.

Purpose of the Study:

  • To apply CRISPRoff and CRISPRon systems in a fungal species for the first time.
  • To investigate the efficacy of CRISPRoff-mediated gene silencing in Aspergillus niger.
  • To assess the reversibility of gene silencing using the CRISPRon system.

Main Methods:

  • Utilized CRISPRoff system with dCas9 fused to ZNF10 KRAB, Dnmt3A, and Dnmt3L domains for gene silencing.
  • Employed CRISPRon system with dCas9 fused to Tet1 catalytic domain for DNA demethylation and gene reactivation.
  • Targeted genes flbA and GFP in Aspergillus niger.

Main Results:

  • Achieved up to 100% gene inactivation of flbA and GFP using CRISPRoff.
  • Observed stable gene silencing through conidiation cycles, even after plasmid removal.
  • Demonstrated full reactivation of flbA using CRISPRon, restoring wildtype phenotype.

Conclusions:

  • CRISPRoff and CRISPRon systems are effective for programmable epigenetic gene control in Aspergillus niger.
  • These systems provide stable and reversible gene silencing, facilitating gene function studies.
  • The successful application in fungi expands the utility of CRISPR-based epigenetic tools.