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Related Concept Videos

Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

1.0K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
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Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

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Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
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Related Experiment Video

Updated: Jul 31, 2025

Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics
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Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics

Published on: August 16, 2024

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Process engineering of natural killer cell-based immunotherapy.

Pedram Motallebnejad1, Anne Kantardjieff2, Frank Cichocki3

  • 1Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN 55455, USA.

Trends in Biotechnology
|May 4, 2023
PubMed
Summary

Natural killer (NK) cells are a promising cancer therapy due to their cytotoxic and allogeneic potential. Manufacturing processes for these off-the-shelf cell therapies are distinct from traditional autologous treatments.

Keywords:
biomanufacturingbioreactornatural killer cellprocess analytical technologyquality by designscale-up

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Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells
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Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

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Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells
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Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

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Area of Science:

  • Immunology
  • Cellular Therapy
  • Biotechnology

Background:

  • T cells are common in cancer therapy, but natural killer (NK) cells offer unique advantages.
  • NK cells possess inherent cancer-killing abilities and are suitable for allogeneic transplantation.
  • Their ability to proliferate and be cryopreserved enables off-the-shelf applications.

Purpose of the Study:

  • To highlight the biological characteristics of NK cells.
  • To review existing protein biologic manufacturing technologies.
  • To discuss adapting these technologies for robust NK cell biomanufacturing.

Main Methods:

  • Review of NK cell biology and proliferation mechanisms.
  • Analysis of protein biologics manufacturing techniques.
  • Exploration of adapting biomanufacturing processes for NK cells.

Main Results:

  • NK cells exhibit potent cytotoxic activity against cancer cells.
  • NK cells can be expanded, cryopreserved, and utilized as readily available treatments.
  • Manufacturing NK cells requires distinct processes compared to autologous cell therapies.

Conclusions:

  • NK cell therapy presents a viable and potentially curative treatment for cancers.
  • Adapting protein biologic manufacturing offers a pathway for scalable NK cell production.
  • Robust biomanufacturing is crucial for the clinical success of allogeneic NK cell therapies.