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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jul 31, 2025

Sample Preparation for Mass Cytometry Analysis

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

Cell Viability Multiplexing: Quantification of Cellular Viability by Barcode Flow Cytometry and Computational

Valentina Giudice^1,2, Victoria Fonseca³, Carmine Selleri⁴

¹Department of Medicine, Surgery and Dentistry "Scuola Medica Salernitana", University of Salerno, Baronissi, Salerno, Italy. vgiudice@unisa.it.

Methods in Molecular Biology (Clifton, N.J.)

|May 4, 2023

Summary

Fluorescent cell barcoding (FCB) enables high-throughput analysis and viability assessment in lymphocytes and monocytes. This protocol details manual and computational methods for FCB, offering optimization and validation guidance for clinical samples.

Keywords:

Fixable dyes Flow cytometry Fluorescent cell barcoding Multiplexing Viability

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Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

Fluorescent cell barcoding (FCB) is a flow cytometry technique for multiplexed analysis.
FCB minimizes technical variations after protocol optimization and validation.
FCB is commonly used for protein phosphorylation analysis and cellular viability assessment.

Purpose of the Study:

To describe a protocol for fluorescent cell barcoding (FCB) combined with viability assessment.
To apply FCB to lymphocytes and monocytes using manual and computational analysis.
To provide recommendations for FCB protocol optimization and validation for clinical samples.

Main Methods:

Protocol description for FCB combined with viability assessment.
Application of FCB to human lymphocytes and monocytes.
Utilized both manual and computational analysis methods.
Included recommendations for protocol optimization and validation.

Main Results:

Detailed protocol for FCB and viability assessment on lymphocytes and monocytes.
Demonstrated utility of FCB for multiplexed analysis and viability.
Provided guidance for optimizing and validating FCB protocols for clinical use.

Conclusions:

FCB is a versatile technique for high-throughput cellular analysis, including viability.
The described protocol offers a framework for applying FCB to clinical samples.
Optimization and validation are crucial for reliable FCB results in clinical settings.