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Assaying Spontaneous Network Activity and Cellular Viability Using Multi-Well Microelectrode Arrays.

Seline S Choo1,2, Jackson Y Keever2,3, Jasmine Brown2,4

  • 1Oak Ridge Institute for Science and Engineering, Oak Ridge, TN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 4, 2023
PubMed
Summary

Microelectrode array (MEA) technology enables neural network activity measurement. Novel methods allow repeated cell health assessments using electrical impedance, complementing traditional viability assays for comprehensive chemical effect analysis.

Keywords:
Alamar blueCytotoxicityElectrical impedanceLactate dehydrogenaseMicroelectrode array (MEA)Multiplexed assaysNeural network formationViability

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Area of Science:

  • Neuroscience
  • Biotechnology
  • Toxicology

Background:

  • Microelectrode array (MEA) technology measures neural activity.
  • Traditional cell viability assays (LDH, CTB) are endpoint measurements.
  • Existing methods limit assessing cell health during network development.

Purpose of the Study:

  • To introduce multiplexed methods for assessing neural network function and cell health.
  • To describe rapid, repeated cell health assessments using electrical impedance.
  • To detail procedures for acute and network formation screening.

Main Methods:

  • Utilizing microelectrode array (MEA) for spontaneous and evoked neural activity measurement.
  • Employing a multiplexed approach to determine cell viability alongside network function.
  • Measuring electrical impedance of attached cells for real-time cell health assessment.
  • Integrating lactate dehydrogenase (LDH) and CellTiter-Blue® (CTB) assays.

Main Results:

  • Electrical impedance provides a non-destructive, repeated measure of cell attachment and health.
  • Multiplexed assays allow simultaneous evaluation of neural function and cell viability.
  • The described methods enable longer exposure assays without compromising cell health.

Conclusions:

  • Electrical impedance offers a valuable addition to MEA assays for monitoring cell health.
  • Multiplexed approaches enhance the efficiency and comprehensiveness of neurotoxicity screening.
  • These methods facilitate a deeper understanding of chemical effects on developing neural networks.