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Related Experiment Video

Updated: Jul 31, 2025

Live Cell Imaging during Mechanical Stretch
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Live Cell Imaging during Mechanical Stretch

Published on: August 19, 2015

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Quantitative Live-Cell Ca2+ Imaging During Isotropic Cell Stretch.

Ulrike Schöler1, Anna-Lena Merten2, Sebastian Schürmann2

  • 1Institute of Medical Biotechnology, Department of Chemical and Biological Engineering, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany. ulrike.schoeler@fau.de.

Methods in Molecular Biology (Clifton, N.J.)
|May 4, 2023
PubMed
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This study introduces a new method combining calcium (Ca2+) imaging with mechanical cell stretching. This technique allows researchers to observe how cells respond to physical forces in real-time, aiding in the study of mechanotransduction.

Area of Science:

  • Cell Biology
  • Biophysics
  • Physiology

Background:

  • Cell viability depends on adapting to mechanical stimuli.
  • Mechanotransduction, the cellular response to mechanical forces, is a growing research area.
  • Calcium ions (Ca2+) are key signaling molecules in cellular processes, including mechanotransduction.

Purpose of the Study:

  • To present a protocol for real-time Ca2+ imaging during mechanical cell stimulation.
  • To enable the study of cellular responses to mechanical forces at the single-cell level.
  • To facilitate functional screening of mechanosensitive ion channels and drug discovery.

Main Methods:

  • Utilizing Ca2+ imaging with a cell stretching device (IsoStretcher).
  • Growing cells (BJ fibroblasts) on elastic membranes for in-plane stretching.
Keywords:
Calcium imagingCell stretchingImage processingIsoStretcherMechanobiologyMechanosignalingMechanotransduction

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  • Employing fluorescent calcium indicator dyes for online, single-cell Ca2+ level monitoring.

    • Main Results:

    • Demonstrated a protocol for live Ca2+ imaging under mechanical stress.
    • Showcased the application of the method for screening mechanosensitive ion channels.
    • Validated the protocol using BJ cells, a fibroblast line sensitive to mechanical stimulation.

    Conclusions:

    • The developed protocol provides novel insights into mechanical regulation of cellular Ca2+ signaling.
    • This method is valuable for investigating mechanotransduction pathways.
    • The technique supports functional screening and drug discovery for mechanosensitive targets.