Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

4.1K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.1K
Understanding Sleep01:11

Understanding Sleep

441
Sleep, an essential biological state, involves significant reductions in physical activity, sensory awareness, and interaction with the environment. This complex physiological process is primarily regulated by specific brain regions, notably the hypothalamus and pons, which govern the sleep-wake cycle or circadian rhythm.
The circadian rhythm, a nearly 24-hour cycle, is deeply influenced by environmental light cues. Light exposure directly affects the hypothalamus, which in turn regulates...
441
Management of Insomnia01:19

Management of Insomnia

289
The sleep cycle, an integral part of human health, consists of several stages with distinct characteristics and functions. It begins with a transition from wakefulness to sleep, known as the light sleep phase, followed by the restorative deep sleep phase, essential for physical recovery and growth. The cycle concludes with the Rapid Eye Movement (REM) phase, characterized by high brain activity and vivid dreaming. Insomnia, a prevalent sleep disorder, involves difficulty falling asleep, staying...
289
Sedatives and Hypnotics Drugs: Miscellaneous Agents01:17

Sedatives and Hypnotics Drugs: Miscellaneous Agents

206
Sedatives and hypnotics encompass a wide range of substances, each with its unique mechanism of action, uses, and potential adverse effects.
Melatonin congeners like ramelteon (Rozerem) and tasimelteon (Hetlioz) selectively bind to melatonin receptors (MT1 and MT2) and thus mimic the actions of melatonin, a hormone that regulates sleep-wake cycles. Tasimelteon is primarily used for non-24-hour sleep-wake disorder, common in blind patients. They are also used to treat conditions like insomnia...
206
Sleep-Wake Cycles01:24

Sleep-Wake Cycles

1.4K
Sleep is an essential physiological process vital to maintaining overall well-being. The reticular activating system (RAS), a network of neurons in the brainstem, regulates wakefulness and sleep. While it may seem passive, sleep consists of distinct cycles, each with its unique characteristics and functions. Two key sleep phases are non-rapid eye movement (NREM) and  rapid eye movement (REM).
NREM Sleep
NREM sleep comprises four progressive stages that seamlessly merge:
1.4K
Substance Use Disorders Affecting Sleep01:24

Substance Use Disorders Affecting Sleep

201
Substance use disorders involve a pattern of using drugs more extensively than intended and continuing use despite harmful consequences. This includes legal substances like alcohol and nicotine, as well as illegal drugs. These disorders often involve both physical and psychological dependence, reflecting compulsive use of substances that significantly alter thoughts, feelings, and behaviors, contributing to a major public health issue.
Understanding the concepts of physical dependence,...
201

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DeepECG.ai: An AI-enhanced ECG analysis platform to bridge the expertise gap from primary care to cardiology.

Journal of electrocardiology·2026
Same author

Rhythmic and arrhythmic components from local-field potentials during non-rapid eye movement sleep in younger and older mice.

Sleep advances : a journal of the Sleep Research Society·2026
Same author

Foundation models for electrocardiogram interpretation: clinical implications.

European heart journal·2026
Same author

Electrocorticographic, astrocytic and transcriptomic signatures in the triple transgenic mouse model of Alzheimer's disease submitted to stearoyl-CoA desaturase inhibition.

Neuropharmacology·2026
Same author

Towards standardizing mitral transcatheter edge-to-edge repair with deep-learning algorithm: a comprehensive multi-model strategy.

Frontiers in network physiology·2025
Same author

"A Responsible Framework for Applying Artificial Intelligence on Medical Images and Signals at the Point of Care: The PACS-AI Platform [Canadian Journal of Cardiology Volume 40, Issue 10, October 2024, Pages 1828-1840]".

The Canadian journal of cardiology·2025

Related Experiment Video

Updated: Jul 31, 2025

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice
08:58

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice

Published on: June 19, 2019

9.8K

Probing pathways by which rhynchophylline modifies sleep using spatial transcriptomics.

Maria Neus Ballester Roig1,2, Tanya Leduc1,2, Julien Dufort-Gervais2

  • 1Department of Neuroscience, Université de Montréal, Montréal, QC, H3T 1J4, Canada.

Biology Direct
|May 4, 2023
PubMed
Summary

Rhynchophylline (RHY) enhances slow wave sleep and alters brain oscillations, with effects varying by time of day and sex. This natural compound impacts gene expression in specific brain regions, offering insights into sleep regulation.

Keywords:
Electrocorticographic oscillationsHypothalamusMolecular profilingSexSleep inductionSlow wave sleep

More Related Videos

Polygraphic Recording Procedure for Measuring Sleep in Mice
08:45

Polygraphic Recording Procedure for Measuring Sleep in Mice

Published on: January 25, 2016

23.9K
The Sleep Nullifying Apparatus: A Highly Efficient Method of Sleep Depriving Drosophila
06:06

The Sleep Nullifying Apparatus: A Highly Efficient Method of Sleep Depriving Drosophila

Published on: December 14, 2020

3.5K

Related Experiment Videos

Last Updated: Jul 31, 2025

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice
08:58

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice

Published on: June 19, 2019

9.8K
Polygraphic Recording Procedure for Measuring Sleep in Mice
08:45

Polygraphic Recording Procedure for Measuring Sleep in Mice

Published on: January 25, 2016

23.9K
The Sleep Nullifying Apparatus: A Highly Efficient Method of Sleep Depriving Drosophila
06:06

The Sleep Nullifying Apparatus: A Highly Efficient Method of Sleep Depriving Drosophila

Published on: December 14, 2020

3.5K

Area of Science:

  • Neuroscience
  • Pharmacology
  • Sleep Medicine

Background:

  • Rhynchophylline (RHY) is an alkaloid from Uncaria plants used in traditional medicine.
  • Uncaria and RHY are known to induce sleep, but mechanisms are unclear.
  • The impact of RHY on brain oscillations during sleep is unknown.

Purpose of the Study:

  • To define RHY's effects on sleep architecture and brain oscillations over 24 hours.
  • To identify the molecular mechanisms underlying RHY's actions.
  • To investigate time-dependent and sex-specific effects of RHY.

Main Methods:

  • Mice received systemic RHY injections at different times.
  • Electrocorticography (ECoG) recorded vigilance states and brain activity.
  • Spatial transcriptomics analyzed gene expression changes in the brain.

Main Results:

  • RHY increased slow wave sleep (SWS) and modulated paradoxical sleep (PS) timing.
  • Brain oscillations during wakefulness and SWS were altered by RHY in a time-dependent manner.
  • RHY affected gene expression related to cell movement, apoptosis, and sleep regulation (e.g., Hcrt, Pmch) in specific brain regions.

Conclusions:

  • RHY influences sleep architecture and brain oscillations.
  • Molecular mechanisms involve region-specific gene expression changes.
  • Findings support RHY's sleep-inducing properties and offer insights for sleep medicine.