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Microbe-Responsive Proteomes During Plant-Microbe Interactions Between Rice Genotypes and the Multifunctional
Denver I Walitang1,2, Aritra Roy Choudhury3, Parthiban Subramanian4,5
1Department of Environmental and Biological Chemistry, Chungbuk National University, 28644, Cheongju, Republic of Korea.
Rice (New York, N.Y.)
|May 5, 2023
Summary
Methylobacterium oryzae CBMB20 colonization in rice induces dynamic proteomic changes, promoting plant growth. These genotype-specific responses involve shifts in metabolic processes and photosynthesis, highlighting key proteins for plant development.
Area of Science:
- Plant-microbe interactions
- Proteomics
- Rice biology
Background:
- Rice is colonized by plant growth-promoting bacteria like Methylobacterium, which influence its development.
- Understanding the molecular mechanisms of these microbe-driven interactions is crucial for agricultural applications.
- Proteomics offers a powerful approach to study dynamic changes in rice during plant-microbe associations.
Purpose of the Study:
- To investigate the proteomic responses of two rice genotypes (IR29 and FL478) upon colonization by Methylobacterium oryzae CBMB20.
- To identify differentially abundant proteins (DAPs) and their associated gene ontology (GO) terms.
- To elucidate the molecular mechanisms underlying Methylobacterium-mediated rice growth promotion.
Main Methods:
- Proteomic analysis of rice (IR29 and FL478) with and without M. oryzae CBMB20 inoculation.
- Identification and quantification of proteins using mass spectrometry.
- Differential protein abundance analysis and Gene Ontology enrichment analysis.
Main Results:
- A total of 3908 proteins were detected, with high similarity between non-inoculated rice genotypes.
- M. oryzae CBMB20 colonization induced dynamic proteomic shifts specific to each rice genotype.
- Key proteins like peptidyl-prolyl cis-trans isomerase, thiamine thiazole synthase, and alanine-tRNA ligase were upregulated, indicating growth promotion mechanisms.
Conclusions:
- Methylobacterium oryzae CBMB20 interaction with rice leads to dynamic, genotype-specific proteomic alterations.
- These changes enhance proteins involved in photosynthesis, metabolism, and cell development, supporting host plant growth.
- Identified proteins provide insights into CBMB20's role in rice development and stress response.
