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Characterization and application of a novel pest-inducible promoter, OsCYP92C21, in conferring resistance to striped stem borer and brown planthopper in rice.

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A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
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Co-Expression Network Analysis: A Future Approach for Pest Control Target Discovery.

Zaihui Zhou1,2, Zhuotian Yao3, Mostafa Abouzaid3

  • 1National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China.

Journal of Agricultural and Food Chemistry
|May 5, 2023
PubMed
Summary

Researchers identified a new gene, Nieman-Pick type C1 homolog b (NPC1b), crucial for cholesterol absorption in the striped stem borer (SSB). This discovery offers a novel target for RNA interference (RNAi) pest control strategies.

Keywords:
CsNPC1bRNA-seqWGCNAstriped stem borer

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Area of Science:

  • Agricultural Entomology
  • Molecular Biology
  • Insect Pest Management

Background:

  • The striped stem borer (SSB) is a significant global rice pest.
  • RNA interference (RNAi) using double-stranded RNAs (dsRNAs) is a promising pest control method.
  • Identifying novel essential genes for RNAi targets is critical for effective pest management.

Purpose of the Study:

  • To discover novel target genes for pest control in the striped stem borer using RNA-Seq data.
  • To investigate the role of Nieman-Pick type C1 homolog b (NPC1b) in insect physiology.
  • To evaluate the utility of Weighted Gene Co-expression Network Analysis (WGCNA) for identifying pest targets.

Main Methods:

  • Diet-based RNA-Seq data was analyzed using Weighted Gene Co-expression Network Analysis (WGCNA).
  • Correlation analysis was performed to identify genes associated with hemolymph cholesterol levels and larval size.
  • Functional characterization of the identified gene (CsNPC1b) was conducted.

Main Results:

  • Nieman-Pick type C1 homolog b (NPC1b) was identified as a highly correlated gene with hemolymph cholesterol and larval size.
  • CsNPC1b expression was linked to dietary cholesterol uptake and insect growth.
  • The study confirmed the critical role of NPC1b in intestinal cholesterol absorption in lepidopteran insects.

Conclusions:

  • NPC1b plays a vital role in cholesterol absorption and insect growth in lepidopteran species.
  • The WGCNA approach is effective for discovering new RNAi targets in insect pests.
  • Targeting NPC1b presents a potential strategy for controlling the striped stem borer.