Related Experiment Video
Updated: Jul 31, 2025
05:30
Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
Published on: January 31, 2025
530
Quantitative proteomics profiling of plasma from children with asthma
Ying Zhou1, Shougang Kuai2, Ruilin Pan3
1Department of Pediatrics Laboratory, The Affiliated Wuxi Children's Hospital of Jiangnan University, Wuxi 214023, Jiangsu Province, China.
International Immunopharmacology
|May 5, 2023
Summary
Researchers identified potential blood biomarkers for pediatric asthma by analyzing plasma proteins in children. Key proteins like AACT, IgA, SAA, and HBB may aid in diagnosing childhood asthma.
Area of Science:
- Proteomics
- Biomarker Discovery
- Pediatric Medicine
Background:
- Asthma control is hindered by the absence of validated blood diagnostic markers.
- Identifying reliable biomarkers is crucial for accurate diagnosis and management of pediatric asthma.
Purpose of the Study:
- To profile plasma proteins in children with asthma.
- To identify potential protein biomarkers for asthma diagnosis.
Main Methods:
- Quantitative proteomics using tandem mass tag (TMT)-labeling.
- Validation of candidate biomarkers via liquid chromatography-parallel reaction monitoring (PRM)/mass spectrometry (MS) and enzyme-linked immunosorbent assay (ELISA).
- Analysis of plasma samples from children with acute exacerbation, clinical remission, and healthy controls.
Main Results:
- 347 differentially expressed proteins were identified between study groups.
- Gene ontology analysis highlighted immune response and extracellular region pathways.
- Seven proteins (AACT, IgA, SAA, HBB) were validated as potential biomarkers for pediatric asthma.
Conclusions:
- This study provides a comprehensive analysis of plasma protein alterations in pediatric asthma.
- Identified proteins may serve as a panel for the accessory diagnosis of childhood asthma.
- Further research can validate these findings for clinical application.
More Related Videos
Related Concept Videos
01:33Proteomics
7.6K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.6K
01:33Peptide Identification Using Tandem Mass Spectrometry
6.6K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.6K