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Related Concept Videos

iPS Cell Differentiation01:22

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The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
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Tissue Renewal without Stem Cells01:23

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After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
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Diabetes Mellitus: Overview and Type I Subtype01:22

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Diabetes mellitus is a chronic metabolic disorder characterized by high blood glucose levels due to inadequate insulin production, insulin resistance, or both. The condition affects millions worldwide and can significantly impact their health and quality of life.
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Induced Pluripotent Stem Cells01:13

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Stem Cell Therapy for Tissue Regeneration01:21

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Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
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The two main cell...
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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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Related Experiment Video

Updated: Jul 31, 2025

Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice
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Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes.

Nathaniel J Hogrebe1, Matthew Ishahak1, Jeffrey R Millman2

  • 1Division of Endocrinology, Metabolism and Lipid Research, Washington University School of Medicine, MSC 8127-057-08, 660 South Euclid Avenue, St. Louis, MO 63130, USA.

Cell Stem Cell
|May 5, 2023
PubMed
Summary
This summary is machine-generated.

Human pluripotent stem cells can generate insulin-producing cells for diabetes treatment. Advances focus on scalable manufacturing, enhanced functionality, and ensuring transplanted stem cell-derived islet survival and safety.

Keywords:
CRISPRSC-isletsbioreactorsclinical trialscritical quality attributescryopreservationdiabetesdifferentiationdistributionencapsulationimmune toleranceinsulinpancreassafetyscale-upsingle-cell sequencingstem cellsstresstherapytransplantation

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Area of Science:

  • Regenerative Medicine
  • Endocrinology
  • Stem Cell Biology

Background:

  • Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia.
  • Current treatments for type 1 diabetes involve lifelong insulin replacement therapy.
  • Human pluripotent stem cells (hPSCs) offer a promising source for generating insulin-producing beta cells.

Purpose of the Study:

  • To review recent advances in generating functional stem cell-derived islets (SC-islets).
  • To discuss strategies for large-scale manufacturing of SC-islets.
  • To highlight methods for improving SC-islet graft viability and safety post-transplantation.

Main Methods:

  • Directed differentiation of hPSCs into pancreatic endocrine cells.
  • Characterization of SC-islet function and maturity.
  • Evaluation of transplantation strategies for graft survival and immune tolerance.

Main Results:

  • Significant progress in generating highly functional SC-islets from hPSCs.
  • Development of scalable manufacturing protocols for SC-islets.
  • Identification of key factors influencing SC-islet survival and integration in vivo.

Conclusions:

  • SC-islets hold potential for unlimited insulin-producing beta cell replacement therapy.
  • Overcoming manufacturing and transplantation challenges is crucial for clinical translation.
  • Continued research is vital for optimizing SC-islet generation and ensuring long-term therapeutic efficacy.