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Updated: Jul 31, 2025

Derivation of Mouse Trophoblast Stem Cells from Blastocysts
Published on: June 8, 2010
Establishment of bovine trophoblast stem cells
Yinjuan Wang1, Hao Ming1, Leqian Yu2
1School of Animal Sciences, AgCenter, Louisiana State University, Baton Rouge, LA 70803, USA.
Abstract:
Here, we report that a chemical cocktail (LCDM: leukemia inhibitory factor [LIF], CHIR99021, dimethinedene maleate [DiM], minocycline hydrochloride), previously developed for extended pluripotent stem cells (EPSCs) in mice and humans, enables de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs retain developmental potency to differentiate into mature trophoblast cells and exhibit transcriptomic and epigenetic (chromatin accessibility and DNA methylome) features characteristic of trophectoderm cells from early bovine embryos. The bovine TSCs established in this study will provide a model to study bovine placentation and early pregnancy failure.
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